There are two main types of characters of the game, Minion costumes and Bosses.

The game allows customization of the Minions. In Minion Rush, players can customize, buy and unlock different costumes for Dave minion. By using different costumes, players can make their minions have better abilities. In addition to Daniel's costume, Sofia minion and Mom minion along with their costumes are also available to purchase by real money.

Players may also be faced with some villains for minions called bosses. Minions should dodge from bosses' attack (including big and small feature objects, and sometimes villains will try to smash minions) or the game ends; on the other hand, if players succeed to throw objects or avoid attacks, the villain will be defeated.